Gonadotropin-releasing peptide from human follicular fluid: isolation, characterization, and chemical synthesis.
نویسندگان
چکیده
منابع مشابه
Gonadotropin-releasing peptide from human follicular fluid: isolation, characterization, and chemical synthesis.
A gonadotropin-releasing peptide has been isolated from human follicular fluid. Its amino acid composition and sequence are completely different from the hypothalamic lutropin-releasing hormone. It is designated human follicular gonadotropin-releasing peptide and abbreviated as hF-GRP. The primary structure of this peptide (H-Thr-Asp-Thr-Ser-His-His-Asp-Gln-Asp-His-Pro-Thr-Phe-Asn-OH) has been ...
متن کاملIsolation and characterization of proteoglycans from human follicular fluid.
Two proteoglycans differing in size and composition were isolated from human follicular fluid. The larger one of high density had a molecular mass of 3.0x10(6) Da, as determined by laser light-scattering, and was substituted with 15-20 chondroitin sulphate (CS) chains (Mr 60000-65000). Half of the CS disaccharides were 6-sulphated, whereas the remaining ones were non-sulphated. Digestion of the...
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Monomer proteoglycan was isolated from porcine ovarian follicular fluid by isopycnic CsCl centrifugation in the presence of 4 M guanidine HCl and protease inhibitors. The elution profile of the D1 preparation on Sepharose 2B was similar to that of monomer proteoglycan from bovine nasal cartilage, indicating a similar molecular size. Follicular fluid proteoglycans consist of about 20% protein, 5...
متن کاملOptimization of The Cell Aggregates Method for Isolation and Purification of Human Granulosa Cells from Follicular Fluid
Objective Aspirated ovarian follicular fluids (FF) contain luteal granulosa cells (LGC) and other contaminating cell types. Several strategies such as the antibody binding methods, the flask method, the cell strainer, and the positive selection of granulosa aggregates after density gradient centrifugation, were tested as LGCs purification methods. Each of these strategies has its own advantages...
متن کاملThe Correlation of the Follicular Fluid Levels of Anti-Mullerian Hormone and Beta Human Chorionic Gonadotropin with Oocyte Matura-tion
Background: Follicular fluid (FF) plays a key role in preparing a microenvironment for the development and fertilization of oocytes. Moreover, biochemical and hormonal changes in the FF surrounding the oocyte can affect its quality and successful fertilization. The present study was conducted to investigate the relationship of beta human chorionic gonadotropin (beta-HCG) and anti-mullerian horm...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1987
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.84.4.959